Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclin E2 antibody (11935-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 11935-1-AP (Cyclin E2 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

IP result of anti-Cyclin E2 (IP:11935-1-AP, 4ug; Detection:11935-1-AP 1:300) with Jurkat cells lysate 4000ug.

1X10^6 HeLa cells were stained with 0.2ug Cyclin E2 antibody (11935-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500.

1X10^6 HeLa cells were intracellularly stained with 0.2 ug Cardinal Red™ Anti-Human Cyclin E2 (CR-11935) (red), or 0.2 ug isotype control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®488 Cyclin E2 antibody (CL488-11935) at dilution of 1:100.

1X10^6 HeLa cells were intracellularly stained with 0.4 ug CoraLite®488 Anti-Human Cyclin E2 (CL488-11935) (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 HeLa cells were intracellularly stained with 0.2 ug CoraLite® Plus 647 Anti-Human Cyclin E2 (CL647-11935) (red), or 0.2 ug Isotype Control (blue). Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite® Plus 647 Cyclin E2 antibody (CL647-11935) at dilution of 1:200.

Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CoraLite®555 Cyclin E2 antibody (CL555-11935) at dilution of 1:100.

Cyclin E fusion protein Ag25403 SDS-PAGE

Cyclin E fusion protein Ag26352 SDS-PAGE

Various lysates were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 11554-1-AP (Cyclin E1 antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

1X10^6 MCF-7 cells were intracellularly stained with 0.4 ug Anti-Human Cyclin E1 (11554-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

1X10^6 HeLa cells were intracellularly stained with 0.4 ug Anti-Human Cyclin E1 (11554-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Isotype Control. Cells were fixed and permeabilized with Transcription Factor Staining Buffer Kit (PF00011).

Non-treated HeLa and nocodazole treated HeLa cells were subjected to SDS PAGE followed by western blot with 11554-1-AP (Cyclin E1 antibody) at dilution of 1:1200 incubated at room temperature for 1.5 hours. The membrane was stripped and re-blotted with GAPDH antibody as loading control.